Staining, Histochemistry and Histotechnology
(Frequently Asked Questions)
Dr. John A. Kiernan
Department of Anatomy
and Cell Biology
The University of Western Ontario
Lidocaine can be added to the fixative during perfusion I would appreciate hearing the Lidocaine concentration again.
This is the recipe for lidocaine I used for perfusion-fixing mormyrids (an electric fish):
Lidocaine (= lignocaine = xylocaine) for use in perfusion fixation (Used to relax blood vessels to permit more complete exchange & infiltration of fixative.):
Lidocaine 50 mg per mL, dissolved 95% EtOH (warm to dissolve). Add slowly to perfusion solution with stirring to make final lidocaine solution concentration wanted (e.g. 1mg/mL = 0.1%)
Note: Do not add the lidocaine directly to the perfusion solution, especially if the solution contains salts! The lidocaine will not go into solution.