Staining, Histochemistry and Histotechnology
(Frequently Asked Questions)
Dr. John A. Kiernan
Department of Anatomy
and Cell Biology
The University of Western Ontario
Solvent to replace xylene AND alcohols
2-butoxyethanol ("Clereum") dehydrating or clearing agent
Decalcification: Acid or EDTA?
Testing for completeness of decalcification
Fatty specimens: Processing into paraffin
Polymethyl methacrylate embedding for bone
Mold release spray
Paraffin processing of skin
Cryoprotection of specimens
Cutting sections of toe or finger nails
Paraffin wax: crystals, additives and cutting
Xylene substitutes: what are they?
Test for water in used absolute alcohol
Molecular sieves for making anhydrous solvent
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