Staining, Histochemistry and Histotechnology
(Frequently Asked Questions)
Dr. John A. Kiernan
Department of Anatomy
and Cell Biology
The University of Western Ontario
histochemistry on cell cultures
How do you perform enzyme histochemistry (NADH Dehydrogenase, succinic Dehydrogenase, cytochrome oxidase)on cultured cells grown on slides? Would you use a detergent (or other means) to permeabilize membranes prior to application of the reaction medium?
I just take the coverglass from the culture medium, give it a rinse in buffer, incubate for required time, wash gently, then mount. No fixing, no detergent; just incubate and mount. It works, so why complicate matters?