Description: 8-OHdG (8-Hydroxy-2’-deoxyguanosine) is suitable for immunohistochemistry in formalin-fixed, paraffin embedded sections. 21 analogues of 8-OHdG were examined with only two exhibiting mini cross-reactivity. 8-sulfhydrylguanosine and 8-OHG demonstrate some cross-reactivity at high concentrations (at least 2 orders of magnitude were required for positive cross-reactivity.
Name: Mouse Anti-8-OHdG Antibody
Clone: Mouse monoclonal
Supplier: Oxis International, Inc.
Catalog Number: 24326
Dilution: 1:20 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.
Incubation Time/Temp: 60 min/room temperature
|Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)|
|Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)|
|Heat/Cool Temperature: 95-100 ºC/room temperature|
|Heat/Cool Time: 20 minutes/20 minutes|
|Standard Method: ABC Method or LSAB Method|
|Enhanced Method: Polymeric Methods|
|Incubation Time/Temperature: 1-3 minutes/room temperature|
|Reagent: Mayer's Hematoxylin|
|Staining Time: 30 seconds|
|Staining Pattern: Nuclear|
|Images: Search image|
|Species Reactivity: Human|
|Fixation: Formalin fixed paraffin sections|
|Positive Control: Skin|
|Negative Control: Omit primary antibody, isotype control, absorption control|
|Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary|