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Annexin V Antibody Staining Protocol for Immunohistochemistry
Description: Annexin V also know as endonnexin II (ENX2）,or placental protein 4 (PP4). Endonexin II is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It binds to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Primary Antibody
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Clone: Rabbit polyclonal |
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Supplier: IHC World |
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Catalog Number: IW-PA1008 |
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Dilution: Ready to Use |
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Incubation Time/Temp: 60 min/room temperature |
Antigen Retrieval
| Device: Incubator |
| Buffer/pH value: IHC-TekTM Proteinase K Solution (Cat# IW-1101) |
| Heat/Cool Temperature: 37 ºC/room temperature |
| Heat/Cool Time: 20 minutes/20 minutes |
Detection Methods
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
Chromogen Substrate
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
Counterstain
| Reagent: Gill's Hematoxylin or Mayer's Hematoxylin |
| Staining Time: 30 seconds |
Results:
| Staining Pattern: Membrane/cytoplasmic |
| Images: Search image |
Additional Information:
| Species Reactivity: Human, mouse, rat, rabbit |
| Fixation: Formalin fixed paraffin sections |
| Positive Control: Rat cardiac muscle |
| Negative Control: Omit primary antibody, isotype control, absorption control |
| Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: