 Methods & Techniques For Histologists and Immunohistochemists |
Bone Morphogenetic Protein 4 (BMP4) Antibody Staining Protocol for Immunohistochemistry
Description: Bone morphogenetic protein 4 (BMP4) is one of nine structurally related BMPs belonging to the transforming growth factor beta (TGF-beta) superfamily of secreted proteins. Mature BMP4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP4 has important roles as a signalling molecule in the developing central and peripheral nervous system, musculature and skeleton. It also participates in a signalling pathway found in insects and worms. BMP also plays an important role in early tooth morphogenesis.
Primary Antibody
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Name: Bone Morphogenetic Protein 4 Antibody |
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Clone: 3H2, Mouse Anti-Human |
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Supplier: Novocastra Labs |
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Catalog Number: NCL-BMP4 |
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Dilution: 1:20 |
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Incubation Time/Temp: 60min/room temperature |
Antigen Retrieval
| Device: Antigen retrieval is not needed |
| Buffer/pH value: N/A |
| Heat/Cool Temperature: N/A |
| Heat/Cool Time: N/A |
Detection Methods
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
Chromogen Substrate
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
Counterstain
| Reagent: Gill's Hematoxylin or Mayer's Hematoxylin |
| Staining Time: 30 seconds |
Results:
| Staining Pattern: Cytoplasmic |
| Images: Search image |
Additional Information:
| Tissue Type: Prostatic adenocarcinoma or osteoclastoma |
| Fixation: Formalin-fixed paraffin sections |
| Positive Control: Prostatic adenocarcinoma or osteoclastoma |
| Negative Control: Omit primary antibody, isotype control, absorption control |
| Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: