Description: Polyclonal Rabbit Anti-Human c-erbB-2 Oncoprotein is intended for use in immunocytochemistry. The antibody labels normal epithelial cells, which, however, generally express c-erbB-2 protein at a very low level. It is a useful tool for the identification of overexpression of cerbB-2 oncoprotein in a variety of epithelial neoplasms, for example subsets of breast carcinomas, pulmonary adenocarcinomas, colorectal adenocarcinomas, pulmonary squamous and gastric adenocarcinomas, transitional cell carcinomas of the urinary bladder, and endometrial adenocarcinomas. Differential identification is aided by the results from a panel of antibodies. Interpretation of results must be made within the context of the patient’s clinical history and other diagnostic tests by a certified professional.
Primary Antibody
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Name: c-erbB-2 Oncoprotein Antibody |
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Clone: Polyclonal, Rabbit anti-Human |
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Supplier: Dako |
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Catalog Number: A0485 |
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Dilution: 1:500 |
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Incubation Time/Temp: 60 minutes/room temperature |
| Device: Steamer |
| Buffer/pH value: Tris-EDTA/pH 9.0 |
| Heat/Cool Temperature: 95-100 ºC/room temperature |
| Heat/Cool Time: 20 minutes/20 minutes |
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
| Reagent: Gill's Hematoxylin or Mayer's Hematoxylin |
| Staining Time: 30 seconds |
| Staining Pattern: Membrane |
| Images: Search image |
| Tissue Type: Breast |
| Fixation: Formalin fixed paraffin sections |
| Positive Control: Breast carcinomas. c-erbB-2 oncoprotein is a normal tissue component and the antibody may display a weak labelling of normal epithelial cells. Squamous epithelium in the esophagus and tonsil may in some cases show moderate staining. Prostatic gland tissue has also been found moderately positive. |
| Negative Control: Omit primary antibody, isotype control or absorption control |
| Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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