CD25 Antibody Staining Protocol for Immunohistochemistry

Description: CD25 antigen, the alpha subunit of interleukin-2 receptor, is a single-chain glycoprotein with a molecular weight of 55kD. Following the activation of T cells with antigen or mitogen in the presence of the monokine interleukin-1, interleukin 2 (IL-2) is rapidly synthesised and secreted. In response to this, a subpopulation of T cells expresses high affinity receptors for IL-2. These cells proliferate, expanding the T cell population which is capable of mediating helper, suppressor and cytotoxic functions. IL-2 receptor is not uniquely found on T cells and is expressed on HTLV-I transformed T and B cells, EBV transformed B cells, myeloid precursors and oligodendrocytes. It is absent on thymocytes, resting T cells, non-activated B cells and null cells.