Description: Melan-A is expressed in the majority of human melanomas as well as in melanocytes. The Melan-A gene, also called MART-1, encodes a melanoma specific antigen recognized by autologous cytotoxic lymphocytes. This antibody reacts with many tumor cells: Benign and malignant adrenocortical tumors, Leydig tumors of the testis, and Sertoli-Leydig ovarian tumors.
Name: Melan-A Antibody
Clone: A103, Mouse anti-Human
Catalog Number: M7196
Dilution: 1:30 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.
Incubation Time/Temp: 60 min/room temperature
|Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)|
|Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)|
|Heat/Cool Temperature: 95-100 ºC/room temperature|
|Heat/Cool Time: 20 minutes/20 minutes|
|Standard Method: ABC Method or LSAB Method|
|Enhanced Method: Polymeric Methods|
|Incubation Time/Temperature: 1-3 minutes/room temperature|
|Reagent: Mayer's Hematoxylin|
|Staining Time: 30 seconds|
|Staining Pattern: Cytoplasmic|
|Images: Search image|
|Tissue Type: Testis tumors, ovarian tumors, CaC1 melanoma cells and Melanomas.|
|Fixation: Formalin fixed paraffin sections, acetone fixed frozen sections, or cytospin preparations.|
|Positive Control: Testis tumors, ovarian tumors, CaC1 melanoma cells and Melanomas.|
|Negative Control: Omit primary antibody, isotype control, absorption control|
|Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary|
Melan-A/MART-1 (Clone M2-7C10 + M2-9E3, Labvision, Cat# MS-716-P1) also works well at 1:100 dilution on paraffin sections with Citrate Buffer Method.