Myosin VIIa Antibody Staining Protocol for Immunohistochemistry

 

Description:  Myosin-VIIa is one of the unconventional members of the myosin molecular motor superfamily that move along filamentous actin. The protein is expressed primarily in the inner ear, retina and testis. Defects in the myosin-VIIa gene (MYO7A) are responsible for Usher syndrome, characterized by hearing impairment, lack of vestibular function and progressive retinal degeneration.MYO7A mutations are are responsible for deafness in shaker-1 mice and in other model systems. In the inner ear, myosin-VIIa is required for assembly of stereocilia, the sensory structures necessary for detecting sound signals. 

 

Primary Antibody

Name: Rabbit Anti-Myosin VIIa Antibody

Clone: Rabbit Polyclonal

Supplier: Proteus Biosciences

Catalog Number: 25-6790

Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature


Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain
Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Results:
Staining Pattern: Cytoplasmic
Images: Search image

Additional Information:
Species Reactivity: Human, Mouse, Rat, Pig, Avian, Amphibian
Fixation: Formalin fixed paraffin sections
Positive Control: Inner ear, retina, testis
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary

 

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