PDGF-BB/PDGFB Antibody Staining Protocol for Immunohistochemistry

 

Description: PDGF-B is the major polypeptide mitogen in serum for cells of mesenchymal origin; it promotes cellular proliferation and inhibits apoptosis. Immunohistochemistry: LS-B4182 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval.

 

Primary Antibody

Name: Goat Anti-PDGFB Antibody

Clone: Goat Polyclonal

Supplier: LifeSpan BioSciences.

Catalog Number: LS-B4182

Dilution: 1:200 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature


Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB Chromogen Substrate
Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain
Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Results:
Staining Pattern: Cytoplasmic
Images: Search image

Additional Information:
Species Reactivity: Human. Predicted Reactivity: Bat, Elephant, Gibbon, Marmoset, Panda, Bovine, Dog, Gorilla, Horse, Monkey, Mouse, Opossum, Rat, Sheep
Fixation: Formalin fixed paraffin sections
Positive Control: Pancreas, placenta
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary

 

References: