Tau Antibody Staining Protocol for Immunohistochemistry


Description: The microtubule-associated proteins (MAPs) coassemble with tubulin into microtubules in vitro. Microtubule-associated protein tau appears to be enriched in axons. Tau is composed of 352 to 441 amino acids. The isoforms differ from each other by the presence or absence of 29-amino acid or 58-amino acid inserts located in the N terminus and a 31-amino repeat located in the C terminus. Tau is important in establishing and maintaining neuronal morphology and is a major component of the neurofibrillary tangles (NFTs) characteristic of Alzheimer's brain. Microtubule-associated protein tau (MTBT1) is mapped to chromosome 17q21.


Primary Antibody

Name: Tau IHC Antibody

Clone: Mouse Monoclonal: TAU-93

Supplier: IHC World

Catalog Number: IW-MA1093

Dilution: Ready to Use

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Cytoplasmic
Images: Search image

Additional Information:
Species Reactivity: Human, mouse, rat.
Fixation: Formalin fixed paraffin embedded tissue sections.
Positive Control: Alzheimer’s brain tissue (neurofibrillary tangles, senile plaques).
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary