TRAP-1 Antibody Staining Protocol for Immunohistochemistry

Description: Transforming growth factor β (TGFβ) receptor associated binding protein
(TRAP-1) participates in the regulation of the TGFβ signaling pathway. TGFβ is a secreted ligand that induces transcription of various targeted genes involved in cell proliferation, differentiation and apoptosis by sequentially binding to surface TGFβ type II receptors and inducing the autophosphorylation of the type II receptor and the transient transactivation of the type I TGFβ receptor. The signal is then propagated through the Smad family of transcription factors, which leads to the expression of the targeted genes. The cytosolic TRAP-1 protein selectively associates with the phosphorylated type I TGFβ receptors, but not with the unphosphorylated type I receptors or type II receptors. TRAP-1 binding to the receptor results in the inhibition of TGFβ signaling, thereby inhibiting the transcription of TGFβ target genes.
The carboxy terminus of TRAP-1 is also able to bind to 5-lipoxygenase, a mediator of lipid metabolism for the production of leukotrienes, where it may then regulate the signaling within leukocytes and other inflammatory mediating cells.