Ubiquitin Antibody Staining Protocol for Immunohistochemistry

 

Description: This polyclonal antibody detects the presence of intracellular ubiquitinated filamentous inclusions in the periphery of senile plaques and neurofibrillary tangles in Alzheimer's disease and lewy bodies in Parkinsion's disease.

 

Primary Antibody

Name: Ubiquitin Antibody

Clone: Rabbit polyclonal

Supplier: Dako

Catalog Number: Z-0458

Dilution: 1:500

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval

Device: Steamer

Buffer/pH value: Citrate Buffer/pH 6.0

Heat/Cool Temperature: 95-100 ºC/room temperature

Heat/Cool Time: 20 minutes/20 minutes

Detection Methods

Standard Method: ABC Method or LSAB Method

Enhanced Method: Polymeric Methods

Chromogen Substrate

Reagent: DAB

Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain

Reagent: Gill's Hematoxylin or Mayer's Hematoxylin

Staining Time: 30 seconds

Results:

Staining Pattern: Cytoplasmic

Images: Search image

Additional Information:

Species Reactivity: Mouse, rat

Fixation: Formalin fixed paraffin sections

Positive Control: Brain tissue of Alzheimer's or Parkinson's disease

Negative Control: Omit primary antibody, isotype control, absorption control

Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary

 

References: