Methods & Techniques
For Histologists and Immunohistochemists
 

Nuclear Fast Red Counterstain Protocol

 

 

 

Nuclear Fast Red (Kernechtrot) Solution

 

       Nuclear fast red ------------------------- 0.1 g

       Aluminum sulfate ------------------------ 5 g

       Distilled water ---------------------------100 ml

       Dissolve aluminum sulfate in water. Add nuclear fast red and slowly heat to boil and cool. Filter and add a grain of thymol as a preservative.

 

Staining Pattern

Nuclear

 

Suggested Use

Counterstaining for immunohistochemistry or other special staining.

 

Suggested Staining Time

5 minutes