Methods & Techniques
For Histologists and Immunohistochemists		  

Enhanced Immunohistochemistry Staining Methods

Polymeric Method: Dako EnVision or Vector ImmPRESS

 

 

 

Dako's EnVision Systems

EnVision Systems are based on dextran polymer technology. This unique chemistry permits binding of a large number of enzyme molecules (horseradish peroxidase or alkaline phosphatase) to a secondary antibody via the dextran backbone. The benefits are many, including increased sensitivity, minimized non-specific background staining and a reduction in the total number of assay steps as compared to conventional techniques. EnVision+ was developed after EnVision to provide increased sensitivity.

Simple Protocol:
  1. Application of primary antibody
  2. Application of enzyme labeled polymer
  3. Application of the substrate chromogen
Vector's ImmPRESS Polymerized Reporter Enzyme Staining System

This system is based on a new method of polymerizing enzymes and attaching these polymers to antibodies. The novel approach employed to form enzyme "micropolymers" avoids the intrinsic shortcomings of using large dextrans or other macromolecules as backbones. Attaching a unique "micropolyer with a high density of very active enzyme to a secondary antibody generates a reagent that overcomes steric interference and provides enhanced accessibility to its target. The result is outstanding sensitivity, signal intensity, low background staining, and reduced non-specific binding.

Simple Protocol
  1. Application of primary antibody
  2. Application of enzyme labeled polymer
  3. Application of the substrate chromogen