Cell Biology Applications of Fluorescence Microscopy

 

Stephen Rogers

Manager, Microscopy Suite, Light and Confocal Microscopy

Imaging Technology Group
Beckman Institute for Advanced Science and Technology
University of Illinois at Urbana-Champaign
405 N. Mathews, Urbana, IL 61801

 

 

 

The purpose of this technical bulletin is to provide an introduction to fluorescence microscopy to newcomers to the technique, and to collect a variety of protocols for specific applications of fluorescence in one place. In this bulletin, I have tried to assemble some of the techniques and lore that I have found particularly useful.

 

Contents

• Introduction

• Fluorescence Microscopy

• Specimen Preparation -Fixation

• Specimen Preparation -Permeabilization

• Specimen Preparation - Blocking

• Specimen Preparation - Mounting Media

• Specimen Preparation - Photobleaching

• Techniques for Staining Specific Cellular Structures

• Sample Fixation Protocols