Barber Lab Protocols

 

Dr. Paul H. Barber, Boston University

 

 

 

Barber Lab Protocols

• DNA Extraction: Chelex

• DNA Extraction: Detailed Chelex Extraction Protocol

• PCR: Diluting dNTPs

• PCR: Doing PCR

• PCR: Making and Diluting TBE

• PCR: Electrophoresis of PCR Products

• PCR: Using the GelDoc System

• PCR: SAP/EXO Cleanup of PCR Products

• PCR: Detailed SAP/EXO Cleanup of PCR Products

• Cloning: Making LB Plates

• Coning: Cloning PCR Produts

• Sequencing: Acrylimide Gel

• Sequencing: Pouring and Running Gels on ABI377

• Sequencing: Cycle Sequencing

• Sequencing: Using Membrane Combs

• Sequencing: Sequencing Cheat Sheet

• Microsatellites: Library Development

• Microsatellites: Running Microsatellites on ABI377