Bird Lab Protocols

Plasmids

• competent cells (Robb)

• plasmid miniprep (Robb)

Proteins

• GST-fusion protein (Robb)

• Protein purification using the Bac system (Helen)

• Western blotting (Eilidh)

• Non-denaturing PAGE (Eilidh)

• MBD refolding (Helle)

Protein / DNA interaction

• GST pulldown (Robb)

• ChIP method (Robb)

• ChIP method (Helen)

• Bandshift method (Helen)

DNA analysis

• Bisulfite sequencing (Jennifer)

• Direct sequencing from colony (Jennifer)

• Methylating DNA with Sss1 methylase [Jennifer]

• LOH analysis (Jennifer)

• Glycosylase assay (Eilidh)

• Annealing oligonucleotides (Eilidh)

RNA analysis

• RNA FISH method 1 (Helen)

• RNA FISH method 2 (Helen)