Cell Culture Protocols

• HMT-3522 Cell Media Composition

• HMT-3522 Cell Density Information

• HMT-3522 Passaging Cells

• HMT-3522 Cell Feeding

• EHS "On Top" Culture

• EHS "3D" Culture

• Methyl Cellulose

• Anchorage Independence Assay using Methyl Cellulose

• Invasion Assay

• Soft Agar Assay for Colony Formation

• Protein Lysis of "3D" Culture using PBS

• Isolating Xenograft Tumor Cells for Tissue Culture or Transplantation

Immunohistochemistry/Immunofluorescence

• BrdU Staining

• Propidium Iodide Staining for DNA Ploidy

• Occludin Staining

• In Situ Hybridization on mammary Sections

Molecular and Biochemical Assays

• FACS Analysis

• RNA extraction

• Chromatin Immunoprecipitation Assay

Mouse Mammary Gland Biology

• Mouse mammary gland wholemounts

• Primary Mammary Epithelial Cell Isolation (From the Mouse Mammary Gland)

• Primary Mammary Epithelial Cell Isolation (From the Mouse Mammary Gland): V.Novaro's protocol