Molecular Biology Protocols for Fellow Lab Rats I have developed most if not all of these protocols while at NIEHS, UNC, or Duke. They all work well for me, but be sure to get additional information if you are not familiar with the Intricacies of them.

Tissue Culture

1. Cell lines and reagents I have used

2. FREEZING CELLS

3. TYPE II CELL PREP FROM RAT LUNGS

4. MONOCYTE ISOLATION

5. PERIPHERAL BLOOD CELL ISOLATION

6. ROSETTE

7. COLORECTAL CELL LINES

8. ISOLATION OF PIG CELLS

Enzymes Stuff

1. COX-2 Semiprotein Preparation

2. Oxygraph Setup

DNA Techniques

1. EMSA

2. PCR VENT TAQ

3. PCR PLATNIUM TAQ

4. SYBR GREEN PCR

5. RT SYBR GREEN

6. Commonly used dual labelled probes.html

7. DIG RNA

8. ELECTROPHORESES

9. LABELING

10. OLIGO PREPERATION

11. SEQUENCING SETUP

12. SEQUENCING GELS

13. TOPO Cloning

14. Transformation

RNA and Reverse Transcription

1. SUPER SCRIPT II RT

2. SUPER SCRIPT RT

3. QIAGEN OMNISCRIPT RT

4. NORTHERN BLOTTING

5. RNA ISOLATION THE OLD FASHION WAY

6. RIBONUCLEASE PROTECTION ASSAY

Proteins

1. VEGF ELISA Using Kit

2. VEGF ELISA Quantikine.pdf file

3. PAGE

4. BCA MICROPLATES

5. Whole Cell Lysates from Monolayer Cultures

6. Cell membrane preparation

7. PAGE NOVEX

8. PROTEIN ISOLATION FROM TISSUE

9. PROTEIN ISOLATION FROM MONOLAYER

10. PROTEIN ISOLATION FROM TRIZOL

11. SDS PAGE

12. Nuclear Extracts

13. WESTERN BLOTTING

In situ

1. tunnel staining for Apoptosis

2. H and E staining

Other Stuff for My Laboratory

1. SUPPLIES

2. LIST OF REAGENTS

3. restriction table.html

4. HPLC