Molecular Biology Protocols for Fellow Lab Rats I
have developed most if not all of these protocols while at NIEHS,
UNC, or Duke. They all work well for me, but be sure to get
additional information if you are not familiar with the Intricacies
of them.
Tissue Culture
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Cell lines and reagents I have used
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FREEZING CELLS
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TYPE
II CELL PREP FROM RAT LUNGS
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MONOCYTE ISOLATION
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PERIPHERAL BLOOD CELL ISOLATION
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ROSETTE
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COLORECTAL CELL LINES
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ISOLATION OF PIG CELLS
Enzymes Stuff
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COX-2 Semiprotein Preparation
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Oxygraph Setup
DNA Techniques
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EMSA
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PCR VENT TAQ
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PCR PLATNIUM TAQ
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SYBR GREEN PCR
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RT SYBR GREEN
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Commonly used dual labelled probes.html
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DIG
RNA
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ELECTROPHORESES
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LABELING
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OLIGO
PREPERATION
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SEQUENCING SETUP
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SEQUENCING GELS
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TOPO Cloning
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Transformation
RNA and Reverse Transcription
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SUPER SCRIPT II RT
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SUPER SCRIPT RT
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QIAGEN OMNISCRIPT RT
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NORTHERN BLOTTING
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RNA
ISOLATION THE OLD FASHION WAY
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RIBONUCLEASE PROTECTION ASSAY
Proteins
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VEGF
ELISA Using Kit
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VEGF ELISA Quantikine.pdf file
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PAGE
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BCA MICROPLATES
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Whole Cell Lysates from Monolayer Cultures
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Cell membrane preparation
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PAGE NOVEX
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PROTEIN ISOLATION FROM TISSUE
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PROTEIN ISOLATION FROM MONOLAYER
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PROTEIN ISOLATION FROM TRIZOL
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SDS PAGE
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Nuclear Extracts
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WESTERN BLOTTING
In situ
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tunnel staining for Apoptosis
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H and E staining
Other Stuff for My Laboratory
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SUPPLIES
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LIST OF REAGENTS
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restriction table.html
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HPLC