Cooper Lab Protocols

Light Microscopy Methods

• Affinity Purification of Antibodies
• Antibody Staining of Yeast
• Antibody Staining of Vertebrate Cells on Coverslips
• Phalloidin Staining of Yeast 
• Phalloidin Staining of Vertebrate

Lab Safety

• NFPA Fire Diamond

Protein Biochemistry Methods

• Actin Purification from Nonmuscle Sources
• Actin Purification - G150 Column
• Actin Prep from Muscle
• CapZ Prep from Muscle
• CapZ Prep from Bacterial Expression (pdf)
• Falling Ball Viscometry
• Pyrene Actin Prep