Devreotes Lab Protocols

• Ammonium Sulfate Binding

• GTPgS Activation of ACA

• CHIFF Preparation

• In vivo Actin Polymerization

• Purification of cAR1 from CHIFFs

• In vitro Actin Polymerization

• MolBioProtocols

• Fluorimeter Start and Shut Down

• Western Blot of cAR1

• FTPSWAP

• Photo affinity Labeling of cAR1

• Spot Screening of Phenotypes

• Preparation of Cells with Upper Band and Lower Band

• Electroporation

• GTP Inhibition of Binding

• Phagocytosis

• Antibody Staining for Gold EM

• Needle Assay for Chemotaxis

• Bead Binding Assay

• Screen of Colonies

• Equilibrium Dialysis Binding

• Random Mutagenesis

• In vivo Activation of ACA

• REMI

• Crac Supernatant Preparation

• In Vivo Translocation of CRAC