• GST Fusion Protein Purification from Yeast : This protocol has be used to assay binding of Sst2-RGS and Gbeta/gamma to Gpa1-GST.

• TAP Fusion Protein Purification from Yeast

• FLAG Fusion Protein Purification from Yeast

• Liquid Beta-galactosidase Reporter Gene Assay: This protocol is used to quantitatively measure the pheromone response over a range of pheromone concentrations.

• Pheromone Halo Assay : This is a bio-assay that measures the responsiveness of cells to a factor pheromone. The assay is easy to conduct and the results are usually unambiguous and highly reproducible.

• SDS Whole Cell Extracts : This is a simple and rapid method for extracting total proteins from Yeast for SDS-PAGE and Western Blotting.

• TCA Whole Cell Extracts : This is a quantitative method for extracting total proteins for Western Blotting, especially good with phospho-MAPK antibodies.

• Sucrose Density Gradient Fractionation of Yeast Membranes : This is an excellent protocol for localization of membrane associated proteins in yeast.

• Beta-galactosidase Filter Assay : This method has been used in our lab to screen for and examine mutants with altered pheromone responses.

• "Lazy Bones" PLATE Transformation: This is a quick method for plasmid transformation into yeast.

• Storing Yeast Strains

• Media Recipies for Yeast

• Preparation of Yeast cells for FACS analysis.

• Plasmid Isolation from Yeast using Qiagen "QiaPrep" Kit.

• Sucrose Density Gradient Fractionation of Yeast Membranes : This is an excellent protocol for localization of membrane associated proteins in yeast.

• Plasmid Library Amplification : This protocol has been used successfully in our lab to amplify a LEU2 CEN library. After several screens we determined that the quality of the amplified library was excellent.

• Paula's Perfect Immunofluorescence Protocol

Other Protocol Pages:

• The TRAFO high efficiency Yeast Transformation Home Page.

• Colony PCR method