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Dunham Lab Protocols
Dr. Maitreya Dunham, Lewis-Sigler Fellow
Lewis-Sigler Institute for Integrative Genomics
Carl Icahn Laboratory, Princeton University
Almost all the microarray protocols have been
updated, either to accommodate Agilent's latest randomization of
their reagents or to circumvent recurring artifacts (the CGH
protocol in particular). Please note that I didn't cut these
protocols from whole cloth. They are mostly derived from the work of
others, plus my own amendments and annotations.
Chemostat Protocols
Microarray Protocols
Protocols are specifically for yeast, but they
also work for other species. Also, the Agilent protocols are for
4x44k arrays, but it turns out all that needs adjusted for the
different platforms is the volume, not the amount of labeled
material. Check the Agilent manuals for recommended volumes.
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Ozone and Cy5 [HTML]
Genotyping Protocols
Yeasty Protocols