Lab Protocols and Recipes

DNA

Cell Culture

• Culturing MEFs

• Calcium Phosphate Coprecipitation

• Harvesting murine bone marrow

• Purification of L-KS+  marrow cells

• Hematopoietic CFC assays

• Retrovirus Production

• STO and ES Cell Culture

• XC assay of MoMLV

Mice

• Complete Blood Cell Counts from Mice

• Genotyping p27 knockout mice

• Tail DNA prep

• Toe ID System

• Using the MPD

• Necropsy Form

• GE Micro CT scans

Histology

• Immunoflourescence of MEFs

• Mouse Necropsy

• Necropsy form

• Paraffin embedding and Sectioning

• Staining Protocols

• H & E staining

• BrdU Staining Histological Sections

• X-gal Staining

• HRP-DAB Immunostaining

• AP-BCIP/NBT Immunostaining

• GFP & BrdU In Situ Immunoflourescence

Product Manuals

• TRIzol reagent

• BioRad Bradford protein assay

• Qiagen RNEasy Mini

Lab Maintenance

• Solutions

Useful Formulae

• Formulae

• Power Curves

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