Gustin Lab Protocols

• Initializing Nikon Microscope

• Prepare DMEM (10L)

• Induction of Nfkappa B with LPS and immunofluorescence assay (IFA)

• Protein assay and measurement of protein content of cells

• Ribomax

• Sequencing Order Form

• DH5alpha

• Infection of HeLa cells with RV14

• Protocols from Kurt Gustin papers

• Empigen Lysis Buffer

• Gel Preparation Protocol

• GelCode Silver Stain

• Split HeLa Cells

• Protein Assay

• Protein Gel

• DMRIE-C

• Western Blot

• Rapid Coomassie Blue Protocol