Heberlein Lab Protocols

 

Dr. Ulrike Heberlein, University of California, San Francisco

 

 

 

Heberlein Lab Protocols

 

Select one of the protocols below to download a pdf version.

 

EQUIPMENT

• How to build an inebriometer PDF (908 KB)

ASSAYS

• Inebriometer use PDF (60 KB)
  Inebriometer cleaning PDF (60 KB)
  Booze-o-mat use: Tracking protocol PDF (84 KB)
  Booze-o-mat use: Video settings PDF (88 KB)
  Ethanol absorption assay PDF (544 KB)
  Protein assay PDF (68 KB)
  Lifespan experiment PDF (68 KB)
  Negative geotaxis "Bang" assay PDF (96 KB)
  Dopamine & 3IY feeding and locomotor assays PDF (76 KB)

HISTOLOGY

• Adult CNS dissection protocol PDF (1.7 MB)
  Larval CNS dissection protocol PDF (188 KB)
  Antibody staining protocol PDF (48 KB)
  Dissection & antibody staining of
  3rd instar larval bodywall or NMJ PDF (568 KB)
  Beta-galactosidase activity staining in CNS PDF (52 KB)
  Frozen head sections PDF (252 KB)

MUTAGENESIS

• Ethyl methanesulfonate (EMS) mutagenesis PDF (116 KB)

• Hydroxyurea ablation of mushroom bodies PDF (84 KB)

PREPS

• Fly food recipe PDF (76 KB)