Hedrick Lab Protocols

• Bone Marrow Chimeras

• CFSE Staining

• Cytotoxicity Assays

• DNA Fragmentation Assays for Apoptosis

• ES Cell DNA Extraction: Tube Method

• Fill-In Reactions

• Low Density Spleen Prep

• Mouse Monocycte Cell Lines

• One-Step Miniprep for Screening by Size

• PCR Method for Genotyping Perforin-Deficient Mice

• Phenol/Chloroform Precipitation of DNA

• Phosphatasing with Shrimp Alkaline Phosphatase (S.A.P.)

• Tissue Culture: Splitting Cells

• Transformation Protocol For DH5 Alpha

• Transformation Protocol For MC1061/P3

• Tail Chop Southern Protocol

• Tail Preps: DNA Isolation From Mouse Tails Without Phenol

• Erk1-/- mouse typing Protocol

• Erk2fn/fn mouse typing Protocol