Hevner Lab Protocols

 

Dr. Robert F. Hevner

University of Washington School of Medicine

 

 

Histology, Immunohistochemistry, and In Situ Hybridization

• Nissl staining
• Antibody handling
• Immunohistochemistry (ABC)
• Immunofluorescence (BrdU/double)
• Cell counting
• In vitro transcription (riboprobe)
• In situ hybridization
• DiI photoconversion

Genotyping, DNA Purification, and PCR

• Extraction of tail DNA
• Bacterial transformation
• DNA Minipreps (QIA spin)
• DNA Minipreps (Wizard Plus)
• DNA Midipreps (Wizard Plus)
• PCR
• PCR Primers

Cell and Tissue Culture

• Cortical cell co-culture
• Forebrain slice culture