Kirschner Lab Protocols

Mass Spectrometry Protocols

• In Gel Trypsin Digestion Procedure

Nucleic Acid Protocols

• Fill In Reactioins using Klenow or T4 Polymerase

• Gene Clean Procedure

• MP Bio's Gene Clean II

• Linearization of Plasmid DNA Procedure

• Plasmid DNA Preparation using Wizard Kit

• Promega's Wizard Miniprep kit

• Dephosphorylating DNA with Alkaline Phosphatase

• Ambion's mMessage mMachine

• Novagen's pET system alt link

• PCDNA 3.1 Map

• Qiagen Hi Speed Plasmid Kit

• Qiagen Plasmid Purification

• Qiagen Miniprep Kit

• Qiagen Quick Manuals (PCR Purif., Nucleotide Removal, and Gel Extraction)

• RNeasy MinElute Cleanup Handbook

• Stratagene QuickChange Manual

• X-Gal Staining

• Embryo RT PCR

Protein Protocols

• BioRad Criterion system Manual

• BioRad Protein Assay

• BioRad trans blot SD Manual

• GE Healthcare ECL plus Manual

• GE Healthcare AKTA Protein Purification Design Book

• GE Healthcare Antibody Purification Book

• GE Healthcare Gel Filtration Book

• GE Healthcare Protein Electrophoresis Book

• Invitrogen Bac to Bac Expression System

• Invitrogen Library Efficiency DH5a Competent Cells

• Invitrogen Max Efficiency DH5a Competent Cells

• Invitrogen Novex Electrophoresis Guide

• Millipore Centricon 70 (large volume spin)

• Millipore Centricon Guide

• Promega Dual Luciferase Manual

• Promega Quick Coupled TNT Manual

• Promega TNT Coupled Retic System

• Promega TNT gold 96 System

• Promega TNT SP6 High Yield System

• Promega TNT Wheat Germ System

• Qiagen's EasyXpress Kit

• Qiagen's QIAexpressionist Kit