Kitto Lab Protocols

 

Dr. Kitto, University of Texas at Austin

 

 

 

Biochemistry

• Reagents and Stock Solutions

• Protein Assays

• Indirect ELISA

• Sandwich ELISA

• Purification

• NuPAGE Gel Electrophoresis

• Lysis of Bacteria by Sonication

• Protein Interaction Analysis Using an IASYS Biosensor

Immunology

• Reagents and Stock Solutions

• General Cell Culture

• Polyclonal Antibody Production

• Monoclonal Antibody Production

• CTL Assays

• FACS

• Freezing and Thawing Mammalian Cells

Microbiology

• Reagents and Stock Solutions

• General Cloning

• PCR

• Ethanol Precipitation of DNA

• Obtaining DNA from Agarose Gels (Paper Slurry Method)

• Electroporation