Klymkowsky Lab Protocols

 

Dr. Mike W. Klymkowsky

Molecular, Cellular & Developmental Biology
University of Colorado

 

 

 

Klymkowsky Lab Protocols

• Immunocytochemistry

• Oocyte isolation

• Embryo injection

• SDS PAGE & Immunoblots

• Neural crest transplants

• 2-Dgels

• 1D IEF analysis

• In situ probe synthesis

• Embryo lysates & Immuno-prepicipitation

• Whole-mount TUNEL

• Wax- & cryosectioning

• Making competant bacteria

• In situ hybridization

• Standard Plasmid minipreps

• Green plasmids

• in vitro protein synthesis

• Xenopus ChIP

• Plasmid construction

• MiniPreps for making RNA

• Dex-regulated proteins
  DNA microarray analyses

• DNA fishing in Xenopus

• Making RNAfrom Xenopus

• Gastromaster animal caps

• Two-color, fluorescent in situ hybridization

• Miscellaneous recipes

• PhotoFishing and IP

• Posters in powerpoint

• zebrafish methods