Chromatin IP's (Meluh)
Chromatin IP's (Megee)
Chromatin IP's (Mechanical Lysis)
Flow Cytometry for Yeast: Cell Preparation
FACS machine instructions
Arresting Yeast
High Efficiency Yeast Transformation (Gietz Long Protocol)
Quick Yeast Tranformation (Gietz Short Protocol)
Yeast Gene Knockouts
Met15
Plasmid Isolation from Yeast
Sporulation
Frogging
Frozen Permanents
Sending Strains
YAC Transfer by KAR1
Microscopy
DAPI Staining of Yeast Cells
FISH for Yeast
Quick Fixation of Yeast Cells for GFP Fluorescence
Indirect Immunofluorescence for Yeast I
Indirect Immunofluorescence for Yeast II: modifications for GFP fix.
Rapid DAPI Screen For Chromosome Missegregation Mutants
Pds1:HA Immunofluorescence
Yeast Chromatin Spreads
Yeast Chromatin Spreads II (Guacci)
Meiotic Spreads
Meiotic Synchrony
DNA/RNA
Best PCR
DNA Fragment Purification: Low Melting Agarose
DNA Fragment Purification: Glass Wool
Isolation of High MW Yeast DNA
Posiblot
SDS Hybridization Solutions for Southerns
Yeast RNA Isolation
Northern Blots
Pulse Field Electrophoresis
Protein
Yeast Protein Prep (No Boil)
Yeast Protein Prep (Boil)
SDS Page (Biorad)
Western
Western Blot with BSA
Carbonate Solution For Western Blot
Silver Stain
Reagents and Equipment
Lab Antibodies: Conc. for Western & IF
ChIP Primers for Yeast Chromosomes
Alpha Factor
Ampicillin
Oxalyticase
Prep of Protein A Sepharose Beads
Prep of Glass Beads for Yeast Lysis
RNAse
Salmon Sperm DNA
SCE
Spectrophotometers: Instructions for Lab and Upstairs
Solutions for Posiblot transfer: neutralization and denaturing
Zymolyase
10X TBE
20X SSPE
EDTA
Media
YPD Plates
YPD Media (1L)
YP Media (Large Batch)
YPG Plates
2X N
2X YP Media
Adenine Stock
2X Agar for bacteria and yeast
B plates
Supplements
20% Dextrose
30% glycerol
Dropout Plates (500 ml)
Dropout Plates (3L)
0.3% KOAc
L-Broth
L-Broth + Ampicillin
L-Plates
M9 Pro Thr Plates (Bacterial Media for Growing pDK plasmids)
Benomyl Plates
5-FOA Plates
G418 YEP plates
G418 Dropout plates
Clonat Plates
Lead Plates
Computer
Making PDF files
Making and Ordering Oligos
Quantitation of EtBr Gels (Image Quant & Excel)
Bacteria
Frozen competent E. coli cells
Electroporating E. coli
Site-directed mutagenesis with oligos
STET preps for rapid recovery of plasmid DNA
Microtubules
Minichromosome-Microtubule Binding Assay
Making Microtubule Seeds
Cycling Tubulin
Mt Staining by Indirect Immunofluorescence
Ron Vale Mt Polymerization Buffer