Lazo Lab Protocols

 

Dr. Gerard R. Lazo, Geneticist, USDA ARS

Western Regional Research Center

Genomics & Gene Discovery Research Unit

 

 

• Company Addresses

• Laboratory Safety

• Media

• Buffers

• Phosphate Buffers

• Stock Solutions

• Antibiotics

• Culture Collection

• Collecting Cotton Tissue

• Tissue Lyophilization

• Genomic DNA Purification

• Cotton DNA Isolation

• Arabidopsis DNA Purification from Nuclei

• Plant DNA Extraction Protocol - Meyerowitz Laboratory

• Plant DNA Extraction Protocol - PMB

• Plant Nuclei DNA Extraction Protocol - DP

• Plant Mitochondrial DNA Extraction Protocol - DP

• Plant Chloroplast DNA Extraction Protocol - DP

• Isolation of Genomic DNA from Azorhizobium caulinodans ORS571

• DNA Extraction from ORS571

• DNA Extraction Procedure for Rhizobium spp.

• General DNA Extraction Procedure for Bacteria

• ReadyAmp™ Genomic DNA Purification System

• Quiagen Preps

• Lessons In Paranoia

• RNA Preparation Methods

• RNA Extraction from Cotton

• Plant RNA from Potato

• Isolation Of RNA From Bacteroids and Bacteria

• Transformation Competent Cells

• Transformation

• One-step Transformation of E. coli

• Transformation of Yeast - LiAc Method

• X-Gal

• Large Scale Isolation of Plasmid and Bacterial DNA

• Alkaline Lysis DNA Purifications from Bacteria

• High-Copy Plasmid Purfication Using The TL-100 Rotor

• Xanthomonas campestris Plasmid DNA Mini-Prep

• Plasmid Isolation - Medium

• Plasmid Isolation - Intermediate

• Plasmid Isolation - Large

• Modified Eckhardt Gel Procedure

• DE52 DEAE Cellulose Phage Lambda DNA Purification Maxi-Prep

• Phage Lambda DNA Purification Maxi-Prep

• TL-100 Phage Purification

• TL-100 Phage Rapid Purification Protocol

• P1 DNA preparation

• P1 DNA PEG precipitation

• Fungal DNA Isolation

• Single-Fly DNA Preps for PCR

• Cesium Chloride Gradients

• Restriction Enzyme Buffer Recipes

• Restriction Enzyme Buffer Mixes

• Restriction Enzyme Digest

• Agarose Gel Electrophoresis

• Molecular Weight Standards

• Methylene Blue DNA Staining

• Glassmilk DNA Purification

• DNA from Low Melting Temperature Agarose

• Precipitating DNA

• Quantifying DNA

• Dephosphorylating DNA

• PCR

• Cloning Blunt-end PCR Fragments

• TA Subloning of PCR Fragments

• PCR site-directed mutagenesis

• Subloning of PCR Fragments

• RT-PCR

• Differential Display-Reverse Transcription-PCR

• Optimized Welsh DDRT-PCR Protocol

• DDRT-PCR Eluting Bands

• Microplate Templates

• PCR Error Rates

• Differential cDNA Screening Procedures

• Southern Transfers

• Southern Transfer - Sponge Method

• Hybridizations

• Hybridization Conditions - Northerns

• Post Hybridization

• DNA Probes

• Radioactive Labelling of DNA

• Randon Primer Oligo-labelling of DNA

• Random Primer Method

• Spin Columns

• PCR Protocol

• PCR Amplification from Microbial Colonies

• RAPDs

• Extra Long PCR (Church Lab)

• Sequencing Protocols

• Exonuclease Protocols

• Misc.

• Mutagenesis

• Equilibrating Phenol

• Siliconizing Glassware

• Preparing Salmon Sperm DNA

• Cleaning Radiation

• Clean-up for Ethidium Bromide

• Removal of CsCl/EtBr Waste

• Arabidopsis Seed Collection

• Arabidopsis Transformation

• Arabidopsis Vacuum Infiltration

• Preparation of DNA from Single Rice Seedlings

• Isolation of RNA and DNA from Rice Seedling Mitochondria

• A simple screning method for transgenic rice tissue based on PCR

• Non-aqueous Method for Purification of Cytoplasmic DNA from Individual Rice Plants for RFLP Analysis

• Enhancement of Division Frequency of Protoplasts and Plant Regeneration in Rice

• Isolation of RNA and DNA from different rice tissues

• Improved histochemical staining for beta-D-glucuronidase activity in monocotyledonous plants

• A rapid, reliable method for preparing somatic chromosomes of rice

• cDNA Topics

• Misc. Methods (DUMP FILE)

• Misc. Stocks

• Tissue Culture Stocks

• Misc. Strains

• A. Thaliana Topics

• Preparation Of Nylon Filters For Dot-blot DNA Hybridization

• Xanthomonas campestris Tri-Parental Mating Protocol

• Agrobacterium-mediated Transformation of Cotton

• MOPS Minimal Medium for Xanthomonas campestris

• Xanthomodadin Extraction from Xanthomonas campestris

• Bacteroid Isolation

• Quick Test For Restriction Enzymes