Minion Lab Protocols

 

Dr. Minion, College of Veterinary Medicine at Iowa State University.

 

 

 

Minion Lab Protocols

 

Proteins

  • SDS-PAGE

  • Silver Stain

  • Western Blotting

    • DNA

  • Agarose gels

  • Plasmid DNA Isolation - alkaline lysis (B&D)

  • Mycoplasma DNA - Agarose method

  • Mycoplasma chromosomal DNA

  • DNA Isolation - general methods

  • DNA Glassmilk Isolation

  • M. hyopneumoniae PCR

  • Solutions

  • Elution of DNA from agarose

  • Kinase reactions

  • Phenol extraction of DNA

  • Precipitation of Nucleic Acids

  • Random libraries

  • Restriction digests

  • SmaI digestions

  • CTAB procedure

    • PCR

  • General PCR methods

  • M. hyopneumoniae PCR

  • Purification of PCR fragments

    • Genetics

  • Conjugation

  • Electroporation of E. coli

  • Overcoming restriction barriers in transformation

  • Media

  • Solutions

    • Immunology

  • ELISA

  • Hybridomas: expanding clones, etc.

  • Antisera

    • Mycoplasma

  • PPLO Broth

  • Other mycoplasma media

  • Acid-adjusted Swine Serum

  • Yeast Extract Recipes

  • DNA in agarose

  • Chromosomal DNA in TE

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