Xenopus Egg Extractology

• Crude Extract Prep for In Vitro Spindle Assembly.

• High Speed Supe Extract Prep

• Crude Extract Immunodepletion

• HSS Extract Immunodepletion

Microtubules

• Tubulin Basics

• Large Scale Tubulin Preparation

• Recycling Tubulin

• Labeling Tubulin and Quantifying Labeling Stoichiometry

• Tubulin Polymerization with GTP/GMPCPP/Taxol

• Preparation of Segmented and Polarity Marked Microtubules

• Microtubule Spindowns for Visual Analysis

• Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC

• Negative Stain Electron Microscopy of Microtubules

• Centrosome Prep

• Microtubule Pelleting

Chromosomes & Kinetochores

• CHO Chromosome Prep

• In Vitro Chromatin and Chromosome Assembly

• Pelleting Assembled Chromatin for Microscopy

Antibodies

• Preparation Of Peptide-KLH Conjugates For Immunization

• Testing Rabbit Bleeds By Elisa

• Coupling Peptides To Resin For Affinity Purification

• Purification Of Anti Peptide Antibody

General Techniques

• Fixation and Immunofluorescence of the Cytoskeleton

• Microinjection of Single Cells in Culture

• In-Gel Tryptic Digest for Mass Spec