Crude Extract Prep for In Vitro Spindle Assembly.
High Speed Supe Extract Prep
Crude Extract Immunodepletion
HSS Extract Immunodepletion
Tubulin Basics
Large Scale Tubulin Preparation
Recycling Tubulin
Labeling Tubulin and Quantifying Labeling Stoichiometry
Tubulin Polymerization with GTP/GMPCPP/Taxol
Preparation of Segmented and Polarity Marked Microtubules
Microtubule Spindowns for Visual Analysis
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
Negative Stain Electron Microscopy of Microtubules
Centrosome Prep
Microtubule Pelleting
CHO Chromosome Prep
In Vitro Chromatin and Chromosome Assembly
Pelleting Assembled Chromatin for Microscopy
Preparation Of Peptide-KLH Conjugates For Immunization
Testing Rabbit Bleeds By Elisa
Coupling Peptides To Resin For Affinity Purification
Purification Of Anti Peptide Antibody
Fixation and Immunofluorescence of the Cytoskeleton
Microinjection of Single Cells in Culture
In-Gel Tryptic Digest for Mass Spec