Dr. Andreas Nebenführ, Ph.D.
Department of Biochemistry, Cellular and Molecular Biology
University of Tennessee
This page lists some standard protocols and recipies used by the Nebenführ lab. Some are very specific to our setup here in Knoxville, others are more generic. We hope that they are also useful for other people.And as always, if you notice a mistake in a protocol, or know of a better way of doing things, please let us know! (Note that all protocols are in PDF format.)
Transformation of E. coli cells (heat shock)
Transformation of Arobacterium tumefaciens (electroporation)