Neri Lab Protocols

 

Dr. Dario Neri

Institute of Pharmaceutical Sciences ETHZ

 

• Electrocompetent E.coli cells

• Bacterial electroporation

• Preparation of media

• Preparation of agar plates

• SDS-PAGE on vertical mini gels

• Western blotting

• Preparation of affinity resins

• Protein purification using the NiNTA resin

• Preparation of phage particles from phage vectors

• PCR

• PCR screening

• Running 2D-PAGE

• Silver staining of 2D-gels

• Coomassie staining of 2D-gels

• Scanning 2D gels

• In-gel digestion of proteins

• SYPRO Ruby Protein Gel Stain

• Affinity measurements by band-shift assay

• Affinity measurements by competition ELISA

• Protein expression in Pichia

• Culturing HEK 293 Cells

• Culturing fibroblast

• Culturing endothelial cells

• Transfection of kidney cell line.

• Generating stable cell lines in HEK293

• Protocols for the preparation of tumour cells for s.c. injections

• Preparation of cryopreserving medium

• Protocols for the preparation of cryotubes of tumour cells

• Protocols for the CAM assay

• Protocols for the immunohistochemistry

• Pellet preparation for the cornea micropocket assay

• DNA sequencing using the ABI Prism sequencer

• ETH-2 library protocols: on-line or in Word format

• Buffers

• Praktikum1

• Praktikum2