Nonet Lab Protocols


Dr. Nonet, Washington University in St. Louis.



  • Cloning from low melting agarose [HTML]

  • DpnI cleavage-mediated Site Directed Mutagenesis [HTML]

  • C. elegans Immunohistochemistry with Bouin's Fixative ...WBG article[HTML], Fixation protocol [HTML]

  • High efficiency competent cells using low temperature growth [HTML]

  • Electrocompetent E. coli frozen [PDF] [HTML]

  • Single Worm PCR [HTML]

  • Standard Osmium/gluteraldehyde fixation for electron microscopy [HTML] [PDF]

  • Recovery of plasmids from dead bacterial frozen stocks [HTML]