Rosen Lab Protocols

 

Jeffrey M. Rosen, Ph.D.

C.C. Bell Professor of Molecular and Cellular Biology

Baylor College of Medicine, Houston, Texas

 

Rosen Lab Protocols

• Adenovirus infection of primary MECs
• Avertin (anesthetic for mice)
• ChIP Assay (Chromatin immunoprecipitation assay)
• Freeze mammary gland pieces (for later transplantation)
• Hematoxylin & Eosin (staining of tissue sections)
• Hormone treatment, acute (2 day E+P) plus BrdU injection
• Immunostaining tutorial
• Immunostaining: dilutions and conditions
• Immunostaining: Representative images
• Immunofluorescence: General protocol
• Immunohistochemistry: General protocol
• Immunohistochemistry: "Milk Method"
• Immunoprecipitation Assay (for Western blotting)
• Masson Trichrome staining (stain for collagen)
• Paraformaldehyde, 4% (tissue fixative)
• Primary MEC isolation (mammary epithelial cells)
• Primary MEC extracts (for Western blots/IP assays)
• Progesterone receptor/BrdU double immunofluoresence
• Retroviral infection of tissue culture cells
• TUNEL assay (in situ detection of apoptotic cells)
• Wholemount staining (hematoxylin staining of whole mammary glands)
• Wholemount staining II (carmine alum staining of mammary glands)
• X-Gal staining of tissue (to detect LacZ expression)
• Bacterial beta-Galactosidase Histochemisty Bible