Santos Lab Protocols

The Santos Lab is happy to offer copies of the various laboratory protocols that we employ. These protocols include, among others, methods of sample preservation, DNA and RNA extraction, buffer recipes, and bacterial cloning. Other protocols will be added to this list in the near future.

• General sample preservation method using DMSO (PDF)

• General DNA extraction method using 2XCTAB (PDF)

• RNA purification from Symbiodinium (PDF)

• Sodium acetate precipitation of DNA (PDF)

• DNA purification method using PEG and TAE (PDF)

• 50X Modified TAE Buffer recipe (PDF)

• E. coli mini-prep protocol (PDF)

• Simple E. coli storage and transport method (PDF)

• f/2 medium recipe and antibiotic cocktail for treating Symbiodinium cultures (PDF)

• DNA fingerprinting using amplified fragment length polymorphisms (AFLPs) (PDF)

• Chloroplast 23S-rDNA hypervariable region genotyping of Symbiodinium (PDF)

• Small volume nuclear 18S-rDNA amplifications of Symbiodinium (PDF)