Schmid Lab Protocols

• Collection of standard methods (from Hannah Neumeier's diploma thesis)

• PCR-cloning

• Northern Blots

• Western Blots (for detection of myc, HA, flag, I-kappa B, GFP, TAK1)

• Co-immunoprecipitation

• Kinase assays

• Transient Transfections (with Lipofectamine)

• Calcium Transfections

• JC1-staining to detect apoptotic cells (via the mitochondrial membrane potential)

• ISNT (In Situ Nick Translation for the morphological detection of apoptotic cells)

• Cell cycle analysis by PI-staining and flow cytometry 

• Yeast-DNA preparation

• Yeast transformation (link to Gietz lab)