Bart’s Cookbook and Lab Protocols

Protein Biochemistry

• Immunoprecipitation (verbose)

• Immunoprecipitation (terse)

• Westerns

• Angiotensin Protein Kinase Assay

• Two dimensional peptide mapping

• Phosphoaminoacid analysis

• CNBr mapping

• V8 partial proteolytic mapping

• Biosynthetic labeling

• Autoradiography and fluorography

Tissue Culture

• How to grow tissue culture cells

• Tissue Culture Medium

• Dishes for growing tissue culture cells

• Trypsinizing cells

• MCBL stock solutions for tissue culture

• Counting cells with the Coulter Counter

• Freezing and Thawing cells

• Infection with Retroviruses