Sosnick Lab Protocols

Protein Purification Protocols

• Purification of Ubiquitin from expression lysates

• Amicon Stir Cell Concentrator

• Hobb's Purification Handbook (AP Biotech, PDF)

Protein Analytical Methods

• SDS-PAGE

Molecular Biology Protocols

• General Molecular Biology Information

• Online Primer Ordering

• Primer Tm Calculator

• Codon Tables

• PCR Standard Taq

• Making Protein Point Mutations: Stratagene Quik Change Mutagenesis Kit

• Alternate protocol from Glick Lab

• Agarose & Acrylamide Gels for DNA separations

• DNA Maxiprep using PEG precipitation 

• DNA Miniprep protocol

• Sequencing DNA (Using Campus Facility)

• Protein Expression and Purification

• Making Bacteria Competent

Recipes

• Growth Media
  Luria Broth (LB)
  Luria Broth Agar Plates
  5x M9 Minimal Media
  Terrific Broth (TB)

• DNA Buffers & Stock Solutions
  6x DNA gel loading buffer
  GTE
  NaOH/SDS Lysis Solution
  PEG Solution
  5M Potassium Acetate Solution, pH 4.8
  SDS Stock Solution
  SDS-PAGE Lower TRIS Buffer (4x)
  50x TAE Buffer
  10x TBE Buffer
  TE