Springer Lab Protocols

LAB COOKBOOK (available in downloadable PDF)

Protein Expression

• Establishment of Stable Transfectant of CHO Lec Cells by Jun Takagi

• Transient Transfection into 293T Cells by Jun Takagi

• Preparing a Selenomethionyl Protein by Sara Lindgren

• K562 Cell Transfection

Protein Purification and Characterization

• Purification of ICAM-1 by Mazen Ferzly

• Preparation of Tonsil Lysate by Mazen Ferzly

• [Sandwish] ELISA for Detecting Secreted ICAM-1 by Chang-Duk Jun

• On-column Protein Refolding

Radioactive Experiments

• Bench Top Radioactive Work Protocol by Mazen Ferzly

• Radioiodination by Jun Takagi

Immunochemical Methods

• Metabolic Labeling and Immunoprecipitation by Jun Takagi

• Semi-Quantitative Measurement of Proteins by Dot Blotting by Jun Takagi

• Purification of mAb (IgG) by Chang-Duk Jun

• Flow Cytometry Analysis by Qing Ma

• Animal Protocol by Qing Ma

• Fluorescently Labeling Antibodies

Cell Biology Protocols

• Chemotaxis Assay by Melissa Swope

• Purification of Human Mononuclear Cells and Neutrophils by Chang-Duk Jun

• Separation of Platelets from Whole Blood by Azucena Salas

• L-selectin-PNAd interactions under flow conditions. Flow Chamber Assay by Azucena Salas