Thomson Lab Protocols

• Media and Reagents

  • Thawing Human ES Cells:

  • Splitting Human ES cells on MEFs

  • Freezing Human ES Cells

  • Matrigel Aliquoting and Plating

    • Aliquoting Matrigel

    • How to Make Matrigel Plates

    • Caring for Cells Growing on Matrigel

  • Splitting Human ES cells on Matrigel

• Splitting Human ES cells in Defined Media

  • Embryonic Bodies

  • General Notes on ES Cell Culture

Mouse Embryonic Fibrolasts (MEFs) Protocols

• Harvesting of MEFs from Murine Embryos

  • Freezing MEF Cells

  • Thawing MEF Cells

  • Splitting MEF Cells

  • Irradiationg & Plating MEF Cells

• iPS Cell Media & Reagents

  • Splitting iPS Cells on MEFs

• iPS Cell Certification