Yeast Lab Manual

 

 

Yeast Lab Manual

1 Yeast as a Model Eukaryote

2 Information on Yeast

3 Yeast Strains

4 Growth and Life Cycles

5 The Yeast Genome

6 Genetic Nomenclature
6.1 Chromosomal Genes
6.2 Mitochondrial Gene
6.3 Non-Mendelian Determinants

7 Genetic Analyses
7.1 Overviews with Examples
7.2 Tetrad Analysis
7.3 Non-Mendelian Inheritance

8 Transformation
8.1 Yeast Vector and DNA Fragments
8.2 Synthetic Oligonucleotides
8.3 Mitochondrial Transformation

9 Yeast Vectors
9.1 YIp Vectors
9.2 YEp Vectors
9.3 YCp Vectors

10 Genes Important for Genetic Studies
10.1 URA3 and LYS2
10.2 ADE1 and ADE2
10.3 GAL1 Promoter
10.4 lacZ and Other Reporters

11 Manipulating the Genome In Vitro with Plasmids
11.1 Cloning by Complementation
11.2 Mutagenesis In Vitro
11.3 Two-step Gene Replacement
11.4 Gene Disruption and One-step Gene Replacement
11.5 Plasmid Shuffle
11.6 Recovering mutant alleles

12 Interactions of Genes
12.1 Heterozygosity and Dominant-negative Mutations
12.2 Intragenic Complementation
12.3 Nonallelic Non-complementation
12.4 Suppressors
12.5 Synthetic Enhancement and Epistatic Relationships

13 Genomic Analysis

14 Analyses with Yeast Systems
14.1 Two-hybrid Systems
14.2 Yeast Artificial Chromosomes (YACs)
14.3 Expression of Heterologous Protein in Yeast

Key Words

Bibliography