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TUNEL Staining of formalin fixed paraffin embedded skin section. TUNEL positive cells in green (FITC). Counterstain with PI (red)

Precision Cryoembedding System

A combination of simple techniques and apparatus which allows the user to embed tissues for frozen section with a level of control and predictability that can surpass even paraffin embedding. By using this system, one can embed multiple blocks very quickly without the needs of using isopentane, liquid nitrogen or dry ice. The embedded tissues will be no crush artifact, precisely flat and precisely oriented therefore less trimming is needed. The method can be easily learned and comfortably performed on a shelf just inside most cryostats.

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We offer the following highly targeted marketing channels:

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Today. I did eNOS staining on paraffin sections with three conditions: 1) no antigen retrieval; 2) with citrate buffer steam method; 3) with proteinase K digestion method.

The best staining pattern was from the “no antigen retrieval method”. I thought each antibody has its best condition and the only way to know it is to test by yourself.

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