Neuroscience Research:

• A triple-labeling method: HRP anterograde tract tracing combined with double immunofluorescent cell staining in developing neural tissue of the rat

• Triple staining of normal and degenerating nervous tissue

• A method for triple fluorescence labeling with Vicia villosa agglutinin, an anti-parvalbumin antibody and an anti-G-protein-coupled receptor antibody

• Triple immunohistochemical staining for bromodeoxyuridine and catecholamine biosynthetic enzymes using microwave antigen retrieval

• Inhibitory neurotransmission in rat spinal cord: co-localization of glycine- and GABAA-receptors at GABAergic synaptic contacts demonstrated by triple immunofluorescence staining

• A triple staining procedure to evaluate phagocytic role of differentiated astrocytes

• Triple staining of brain tissue for neuronal classification

• Heterogeneity of nigral and cortical Lewy bodies differentiated by amplified triple-labeling for alpha-synuclein, ubiquitin, and thiazin red

• Calretinin and calbindin-D28k in dopaminergic neurons of the rat midbrain: a triple-labeling immunohistochemical study

• Calbindin D28k and calretinin in oxytocin and vasopressin neurons of the rat supraoptic nucleus. A triple-labeling immunofluorescence study

• Discriminative staining methods for the nervous system: luxol fast blue--periodic acid-Schiff--hematoxylin triple stain and subsidiary staining methods

Cancer Research:

• Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy

• Triple-staining to identify apoptosis of hepatic cells in situ

• Enzyme triple staining for differentiation of lymphatics from venous and arterial capillaries

• Triple immunofluorescence staining for prediction of relapse in childhood precursor B acute lymphoblastic leukaemia

• Simultaneous triple staining for hypoxia, proliferation, and DNA content in murine tumours

• A combined histochemical and double immunohistochemical labeling protocol for simultaneous evaluation of four cellular markers in restenotic arteries

Others:

• Simultaneous triple-immunogold staining of virus and host cell antigens with monoclonal antibodies of virus and host cell antigens in ultrathin cryosections

• Double and triple staining methods for studying the proliferative activity of human B and T lymphoid cells
• Triple staining of human sperm: technical aspects
• Triple staining for simultaneous visualization of cell types in islet of Langerhans of pancreas. Successive application of argyrophil, aldehyde-fuchsin and lead-hematoxylin stains in a single tissue section
• Five subtypes of type A gamma-aminobutyric acid receptors identified in neurons by double and triple immunofluorescence staining with subunit-specific antibodies
• Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electron microscopical double and triple staining method employing primary antibodies from the same species